Keywords:
image reconstruction techniques, optical tomography, X-ray imaging, principal component analysis
Cone-beam X-ray luminescence computed tomography (CB-XLCT), which has been proposed as a new molecular imaging modality recently, can obtain both anatomical and functional tomographic images of an object efficiently, with the excitation of nanophosphors in vivo or in vitro by cone-beam X-rays. However, the ill-posedness of the CB-XLCT inverse problem degrades the image quality and makes it difficult to resolve adjacent luminescence targets with different concentrations, which is essential in drug delivery and treatment monitoring in vivo. To address this problem, a multi-voltage excitation imaging scheme combined with principal component analysis is proposed in this study. Imaging experiments performed on physical phantoms by a custom-made CB-XLCT system demonstrate that two adjacent targets, with the concentration differences of 50 and 100 mg/ml and an edge-toedge distance of 0 mm, can be effectively resolved.
- Peng Gao
- FMMU, China
- Huangsheng Pu
- FMMU, China
- Junyan Rong
- FMMU, China
- Wenli Zhang
- FMMU, China
- Tianshuai Liu
- FMMU, China
- Wenlei Liu
- FMMU, China
- Hongbing Lu
- FMMU, China
- X. Liu, Q. Liao and H. Wang, Fast X-Ray Luminescence Computed Tomography Imaging, Biomedical Engineering, IEEE Transactions on, vol. 61, pp. 1621-1627, 2014.
- D. Chen, S. Zhu, H. Yi, X. Zhang, D. Chen, J. Liang, and J. Tian, Cone beam X-ray luminescence computed tomography: A feasibility study, MED PHYS, vol. 40, pp. 031111, 2013.
- C. Li, K. Di, J. Bec, and S.R. Cherry, X-ray luminescence optical tomography imaging: experimental studies, OPT LETT, vol. 38, pp. 2339, 1 2013.
- X. Liu, Q. Liao and H. Wang, In vivo x-ray luminescence tomographic imaging with single-view data, OPT LETT, vol. 38, pp. 4530-4533, 2013.
- C.M. Carpenter, C. Sun, G. Pratx, R. Rao, and L. Xing, Hybrid Xray/ optical luminescence imaging: Characterization of experimental conditions, MED PHYS, vol. 37 , pp. 4011, 2010.
- A. Cong and G. Wang, A finite-element-based reconstruction method for 3D fluorescence tomography, OPT EXPRESS, vol. 13, pp. 9847- 9857, 2005.
- H. Gao and H. Zhao, Multilevel bioluminescence tomography based on radiative transfer equation Part 2: total variation and l1 data fidelity, OPT EXPRESS, vol. 18, pp. 2894-2912, 2010.
- H. Pu, W. He, G. Zhang, B. Zhang, F. Liu, Y. Zhang, J. Luo, and J. Bai, Separating structures of different fluorophore concentrations by principal component analysis on multispectral excitation-resolved fluorescence tomography images, BIOMED OPT EXPRESS, vol. 4, pp. 1829, 2013.
- Y. Zhou, H. Guang, H. Pu, J. Zhang, and J. Luo, Unmixing multiple adjacent fluorescent targets with multispectral excited fluorescence molecular tomography, APPL OPTICS, vol. 55, pp. 4843-4849, 2016.
- G. Pratx, C.M. Carpenter, C. Sun, R.P. Rao, and L. Xing, Tomographic molecular imaging of X-ray-excitable nanoparticles, OPT LETT, vol. 35, pp. 3345-7, 2010.
- G. Zhang, F. Liu, J. Liu, J. Luo, Y. Xie, and L. Xing, Cone Beam X-ray Luminescence Computed Tomography Based on Bayesian Method, IEEE T MED IMAGING, pp. 1-1, 2016.
- M. Choi, K. Choi, S. Ryu, J. Lee, and C. Choi, Dynamic fluorescence imaging for multiparametric measurement of tumor vasculature, J BIOMED OPT, vol. 16, pp. 046008-046008, 2011.
- Y. Liu, W. Chen, S. Wang, A.G. Joly, S. Westcott, and B.K. Woo, X-ray luminescence of LaF3: Tb3+ and LaF3: Ce3+, Tb3+ water-soluble nanoparticles, J APPL PHYS, vol. 103 , pp. 063105, 2008.
- X. Liu, Q. Liao, H. Wang, and Z. Yan, Excitation-resolved cone-beam X-ray luminescence tomography, J BIOMED OPT, vol. 20, pp. 70501, 2015..